When the WEIRD SISTERS predicted that MACBETH would become King of Scotland, his wife LADY MACBETH encouraged him to murder KING DUNCAN in order to secure the throne for himself. Whilst she is initially more mentally prepared to commit murder than MACBETH at the outset, as the play progresses we see that MACBETH becomes more inured to his actions, and LADY MACBETH becomes more and more emotionally fragile. In this scene, LADY MACBETH is being observed by her Doctor and Gentlewoman whilst sleepwalking and she is reliving the night when they killed DUNCAN and has his blood all over their hands.